منابع مشابه
Cdc42 contributes to phorbol ester-induced Ca2+-independent contraction of pulmonary artery smooth muscle.
We determined the contribution of the Rho family of low molecular GTP-binding proteins to phorbol ester-induced contraction in swine pulmonary artery smooth muscle. In Ca2+-free medium containing 1 mM EGTA, 12-deoxyphorbol 13-isobutyrate (DPB, 1 microM), a protein kinase C (PKC) activator, elicited sustained contractions, which were not inhibited by treatment with verapamil, a voltage-dependent...
متن کاملIntegrin-linked kinase is responsible for Ca2+-independent myosin diphosphorylation and contraction of vascular smooth muscle.
Smooth muscle contraction is activated by phosphorylation at Ser-19 of LC20 (the 20 kDa light chains of myosin II) by Ca2+/calmodulin-dependent MLCK (myosin light-chain kinase). Diphosphorylation of LC20 at Ser-19 and Thr-18 is observed in smooth muscle tissues and cultured cells in response to various contractile stimuli, and in pathological circumstances associated with hypercontractility. ML...
متن کاملCa2(+)-dependent and independent mechanisms of sustained contraction in vascular smooth muscle of rat aorta.
Norepinephrine (NE), prostaglandin F2 alpha (PG), 12-deoxyphorbol 13-isobutyrate (DPB) and high K+ induced sustained increase in cytosolic Ca2+ [( Ca2+]i) and muscle tension in rat aorta. Verapamil, at the concentration which abolished the high K(+)-induced changes, also abolished the increase in [Ca2+]i due to NE, PG and DPB although contractions were only partially inhibited. Excess EGTA furt...
متن کاملEndogenous Cardiac Troponin T Modulates Ca2+-Mediated Smooth Muscle Contraction
Mechanisms linked to actin filaments have long been thought to cooperate in smooth muscle contraction, although key molecules were unclear. We show evidence that cardiac troponin T (cTnT) substantially contributes to Ca(2+)-mediated contraction in a physiological range of cytosolic Ca(2+) concentration ([Ca(2+)](i)). cTnT was detected in various smooth muscles of the aorta, trachea, gut and uri...
متن کاملNa(+)-Ca2+ exchange, myoplasmic Ca2+ concentration, and contraction of arterial smooth muscle.
Na(+)-Ca2+ exchange is proposed to be an important regulator of myoplasmic intracellular Ca2+ concentration ([Ca2+]i) and contraction in vascular smooth muscle. We investigated the role of Na(+)-Ca2+ exchange in regulating [Ca2+]i in swine carotid arterial tissues that were loaded with aequorin to allow simultaneous measurement of [Ca2+]i and force. Reversal of Na(+)-Ca2+ exchange, by reduction...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m011634200